Semi Prep HPLC Column, Bidentate C8, 4um, 100A. 10mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑SP50 Cogent Bidentate C8™ Semi‑Preparative HPLC Column is a short‑length, large‑diameter purification column designed for high‑throughput sample processing and efficient fraction collection when C18 chemistry is overly retentive. Built on Cogent TYPE‑C™ silica hydride technology, this column provides excellent selectivity, fast equilibration, and very robust column lifetime under repeated semi‑preparative conditions.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than conventional C8 columns, yet less hydrophobic than Bidentate C18™, delivering balanced retention ideal for hydrophobic molecules, metabolites, and amine‑containing analytes when C18 retention is excessive.
With a 10 mm internal diameter and 50 mm length, this column is optimized for large injection volumes and high mass loading, enabling rapid purification cycles and efficient collection of target fractions.
System Note: A specialized semi‑preparative HPLC system (appropriate flow‑rate capability, injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size.
Why Choose the 10 × 50 mm Semi‑Prep Bidentate C8™ Format
This configuration is typically selected when:
C18 provides excessive retention for target compounds
High‑throughput purification is required
Large sample volumes must be processed efficiently
Hydrophobic or basic analytes require balanced retention
Fast cycle times are more important than maximum resolution
The short column length enables rapid run times, while the TYPE‑C™ silica hydride chemistry ensures stability and reproducibility across purification workflows.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase surface chemistry
Reduced silanol activity and improved peak shape
Faster equilibration than conventional preparative C8 columns
Very robust lifetime for repeated purification runs
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention for prep applications
Ideal when C18 is overly retentive
Semi‑Preparative Geometry for Throughput
10 mm ID × 50 mm length
High sample loading capacity
Short cycle times for efficient purification
Broad Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Suitable for demanding purification conditions
Typical Applications
Semi‑preparative purification
Fraction collection of hydrophobic compounds
Metabolite isolation
Amine‑containing analytes
Methods where C18 retention is excessive
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable